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EZ-Link （tm） Sulfo-NHS-SS-Biotin, 100 mg介紹

Thermo Scientific BCA蛋白定量產品可以用于研究蛋白：蛋白間相互作用，衡量親和層析后的組分含量，估算從細胞裂解液里提取的膜蛋白含量和高通量篩選融合蛋白。

EZ-Link （tm） Sulfo-NHS-SS-Biotin, 100 mg現(xiàn)貨供應

品牌介紹

美國Pierce公司（www.pierce.com）是蛋白質化學、免疫學和層析等很多方面的和*。http://www.thermo.com.cn/Category828.html

主要產品包括：

1）化學發(fā)光體和比色底物

2）分析試劑

3）熒光標記探針

4）酶聯(lián)免疫分析試劑盒等

僅用于科研

Thermo Scientific EZ-Link Sulfo-NHS-SS-Biotin enables simple and efficient biotin labeling of antibodies, proteins and any other primary amine-containing biomolecules in solution.

Sulfo-NHS-SS-Biotin is a thiol-cleavable amine-reactive biotinylation reagent that contains an extended spacer arm to reduce steric hindrances associated with avidin binding. This reagent is water-soluble, enabling biotinylation to be performed in the absence of organic solvents such as DMSO or DMF for applications that cannot tolerate solvents or are complicated by their inclusion.

Highlights:

• Label almost any protein with biotin to facilitate immobilization, purification or detection
• Ideal for reversible biotinylation; disulfide bond in the spacer arm allows the biotin label to be removed as desired
• Amine reactive Sulfo-NHS ester reacts rapidly with any primary amine-containing molecule to attach the biotin label via a stable amide bond
• Sulfonate group attached to the biotinylation reagent increases water solubility to a minimum of 6 mg/ml, so biotin labeling can be done under physiologic conditions
• Long spacer arm on the biotin reduces steric hindrance when binding to avidin molecules